Unlockable - School Power-up items |
| Edo Ryu | Beat Swordmaster (available for Imperial Side) |
| Spear Ryu (Houzouin Ryu) | Beat Spearmaster (available for Shinsengumi Side |
| Choshu Ryu | Beat Swordmaster (available for Imperial Side |
| Satsuma Ryu (Jigen Ryu) | Beat Swordmaster (available for Imperial Side |
| Hokushin Ittou Ryu | Beat Swordmaster (available both Story Modes) |
| Shintou Munen Ryu | Beat Swordmaster (available both Story Modes) |
| Tama Ryu (Tennen Rishin Ryu) | Beat Swordmaster (Shinsengumi Side before 6th chapter) |
Unlockable - How to unlock characters for 2P mode |
| Nagakura Shinpachi | Beat the last mission on 5th chapter |
| Saigo Takamori | Beat Saigo Takamori's story quest |
| Harada Sanosuke | Beat the last mission on 5th chapter |
| Hirayama Gorou | Kill Hirayama Gorou on the Final mission of 1st chapter |
| Toudou Heisuke | Kill Toudou Heisuke on the last mission of the 4th chapter |
| Itou Kashiitarou | Kill Itou Kashiitarou on the last mission of the 4th chapter |
| Serizawa Kamo | Beat Serizawa Kamo's Side quest |
| Normal bosses | Kill them on normal missions |
| Two Hitokiri | Beat the story quest for two hitokiri |
| Yamazaki Susumu | Start a game on shinsengumi side |
| Sakamato Ryoma | Beat Sakamato Ryoma's story quest |
| Katsura Kogoro | Beat Katsura Kogoro's story quest |
| Tetsunosuke Ichimura | Meet Tetsunosuke Ichimura on the 6th chapter on Shinsengumi side |
| Okita Souji | Beat Okita Souji's story quest |
| Hijikata Toshizo | Beat Hijikata Toshizo's story quest |
| Kondo Isami | Beat Kondo Isami's story quest |
| Yamanami Keisuke | Kill Yamanami Keisuke on the last mission of the 3rd chapter |
| Saito Hajime | Beat Saito Hajime tons of times on Te-awase |
| Shinsengumi Lower Class member | Start a game on the shinsengumi side |
| Takeda Kanryuusai | Complete mission with Takeda Kanryuusai at Masuya |
